The 35-year-old singer closed out her 49-date Formation World Tour at MetLife Stadium in East Rutherford, New Jersey, on Friday, pulling out all the stops for the sold-out crowd with hot new costumes, surprise guests and a debut performance of "6 Inch."
First to join Queen Bey onstage was Serena Williams, taking her place on a regal throne during "Sorry." The queen of the tennis court busted out the same dance moves (twerking and all!) we saw her perform in Beyonce's Lemonade visual.
"Not sorry @beyonce," Williams later wrote on Instagram, sharing a pic of herself in the glitzy costume she rocked.
Fans were in for an even bigger treat when Beyoncé's husband, Jay Z, came onstage for a flirty "Drunk in Love" duet. After Bey exclaimed, "Give it up for my man!" the two shared a steamy smooch, causing the crowd to roar in excitement.
But that's not all. The Texas native also called her pal Kendrick Lamar to the stage, to assist her on "Freedom."
While all eyes were certainly on Beyoncé's guests of honor, it was hard not to notice the sexy new getups she debuted. At one point, she donned a lemonade yellow pantsuit, styled with a bright purple flower necklace. While we wouldn't be surprised to see Bey wear something like this to a red carpet event, it was a rare stage look for the mother of one.
Another memorable costume was her gold mesh T-shirt dress from her own fashion collection, Ivy Park (a similar black version retails for $58). She completed the sporty look with over-the-knee stockings and ankle booties.
One of her most talked about looks, however, was her studded shorts and gold-and-white top combo, which gave off major Michael Jackson vibes. Later, she added an oversized yellow coat to the striking ensemble.
Beyoncé also performed her song "6 Inch" live for the first time on Friday night, which quickly became a trending topic on Twitter. She hung from a harness, spinning around in circles as she belted out the track, which you can see in the video below.
Last month, Beyoncé performed to a star-studded crowd at Dodger Stadium in Los Angeles. Channing Tatum and his wife, Jenna Dewan, were in attendance, as well as Lea Michele, Katy Perry, Gwyneth Paltrow, Christina Aguilera and Jennifer Lawrence.
To see highlights from that show, watch the video below.