"I hear that he gets asked a lot if he is Ruby Rose," she said of her 21-year-old lookalike. "And it must be very frustrating for him."
Not hesitating to make a joke at O'Brien's expense, Rose added, "I feel like you can relate because you look like a mixture of Tilda Swinton, Meryl Streep, and Joel Edgerton."
Being a good sport, the late-night host agreed with the 29-year-old actress. "I like that it's three and two are women," he quipped. "I'm glad that you threw one guy in there. Just to butch it up just a little bit for me."
O'Brien then joked that she should have compared him to Dame Judi Dench and Betty White.
Whether you think there's a resemblance, there's no arguing that Bieber and Rose have become pals. In July, the two were seen comparing arm tattoos while at the trendy sushi restaurant Katsuya in Hollywood, California.
Will Bieber show up to see his lookalike DJ in Las Vegas? Rose starts her residency at XS, Surrender and Tryst Nightclubs in Wynn Las Vegas. Dates include include Wednesday, August 19 during MAGIC Las Vegas, and Sunday, August 23 for the Nightswim at XS.