The multi-talented performer, who has won two Oscars, five Emmys, eight GRAMMYs and a special "Star of the Decade" Tony in 1970, almost sent host James Corden into a fit as she came to the stage.
The 74-year-old theater icon spoke about the tragic shooting in Orlando, Florida during her presentation, sharing, "Tonight our joy is tinged with sorrow. But we're here to celebrate Broadway, and the beauty that artistry can bring into this world."
"I'm very thankful to be in the company of the people in this theater," she continued. "The artists who write the plays, the composers and lyricists who write the songs, and the actors who bring them to life. Art can entertain us and educate us, and in times like these, console us."
Streisand, who then presented the nominees for Best Musical, revealed the winner by referring to her own Revolutionary Era-themed wardrobe, joking, "Thank God I picked the right outfit. The Tony goes to Hamilton."
The producers and cast of the beloved musical, including star and creator Lin-Manuel Miranda, took the stage to accept the award, where producer Jeff Seller spoke about the importance of reaching for your goals and aspirations.
"Alexander Hamilton was a dreamer. George Washington, Eliza Hamilton, Thomas Jefferson were dreamers," Sellers shared. "I stand on this stage tonight, and in this theater, surrounded by dreamers. Dreamers like Lin-Manuel, who had a big idea and worked tirelessly over six years to bring it to life."
After the emotional speech, the cast of Hamilton took the stage to close out the show with a performance (sans costumes) of the girl-powered song "The Schuyler Sisters," starring Renee-Elise Goldsberry, who won one of the show’s 11 Tony Awards on Sunday, taking home the trophy for Best Featured Actress in a Musical. She was joined by Phillipa Soo and Jasmine Cephas Jones.
The Hamilton cast also opened the three-hour awards show with a parody of the song "Alexander Hamilton," introducing Corden to the stage for his epic musical medley monologue.
Among the 11 Tonys that Hamilton took home, two went to Miranda himself for Best Book and Best Original Score. During his acceptance speech, the 36-year-old star recited a moving, emotional sonnet dedicated to his wife and son, which also addressed the Orlando nightclub shooting. Check out the video below to hear his passionate poem.