During the beautiful tribute, which was presented by Darius Rucker and Martina McBride, a photo of Joey appeared on the screen, which immediately received a standing ovation from the star-studded audience.
"A few weeks ago, we lost a member of our country music family with the passing of Joey Feek," Rucker began.
McBride chimed in, saying, "Joey was more than a wonderful singer and an ACM winner along with her husband, Rory -- she was a beloved mom, daughter, family member and friend."
"Of all the different ways we could honor Joey, we think that what would mean the most to Joey and her family is just one more time for all of us -- all of her fans and all of us who loved her -- to give her a big round of applause," Rucker added.
McBride concluded the tribute by sending prayers to Joey's family and friends.
"Joey's daughter Hopie and her friend Julie are here with us tonight," she explained. "God bless them, and God bless you, Joey."
Fans of Joey + Rory immediately took to Twitter to express their thoughts on the "beautiful" tribute, writing things like, "Great to see @joeyandrory honored and loved on at @ACMawards we miss you #joeyfeek," "Water works are about to start" and "All of the applause for the beautiful Joey Feek… tears in my eyes. Such a wonderful woman."