The arresting imagery of her gown echoed the resounding messages of love and acceptance in their heartfelt melody, which was written to benefit the victims of the Pulse nightclub shooting in Orlando, Florida.
After Miranda took the stage to kick off his verse, Lopez even pulled off a mid-song wardrobe change -- unusual for a talk show performance. As cameras came back to Lopez, she was decked out in red, white and blue embroidered bustier.
Miranda, whose Tonight Show appearance came three days after he delivered his farewell performance as the star of the Broadway sensation Hamilton, kept things less formal, rocking his oft-worn "Mr. Write" t-shirt and a "Wash.hts" [Washington Heights] ballcap.
The Roots' bandleader, Questlove, who performed the song with the pair, took to Instagram after to rave about the performance, and to share an amazing shot of Lopez' mindblowing gown.
"I gotta say that #LinManuelMiranda's nerdom is the coolest thing to witness ever. It's so disarming even @JLo was in her most playful mood she's ever been in on our #FallonTonight set," Questlove captioned the pic. "We performed #LoveMakeTheWorldGoRound benefiting our brothers and sisters caught up in the Orlando tragedy. Great moment 2nite. Hope you guys watch it."
Proceeds from the sale of the song, which is available on iTunes, will go directly to the Somos Orlando Fund, which helps provide mental health care and services to the victims of the tragic shooting in Orlando last month.