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Jennifer Lopez and Lin-Manuel Miranda delivered an emotional performance of their powerful new song, "Love Make the World Go Round," on Tuesday's Tonight Show.
Audio from Miranda's emotionally-charged Tony Awards acceptance speech echoed through the theater ("Love is love is love is love is love is love is love, cannot be killed or swept aside,") as the lights came up on Lopez, who took center stage in a colorful, breath-taking gown.
The 46-year-old pop superstar’s stunning dress, with an extremely long train, was made to look like the stained glass windows of a church.
The arresting imagery of her gown echoed the resounding messages of love and acceptance in their heartfelt melody, which was written to benefit the victims of the Pulse nightclub shooting in Orlando, Florida.
After Miranda took the stage to kick off his verse, Lopez even pulled off a mid-song wardrobe change -- unusual for a talk show performance. As cameras came back to Lopez, she was decked out in red, white and blue embroidered bustier.
Miranda, whose Tonight Show appearance came three days after he delivered his farewell performance as the star of the Broadway sensation Hamilton, kept things less formal, rocking his oft-worn "Mr. Write" t-shirt and a "Wash.hts" [Washington Heights] ballcap.
WATCH: Britney Spears, Pink, Gwen Stefani and More Team Up for Musical Tribute to Orlando Victims
The Roots' bandleader, Questlove, who performed the song with the pair, took to Instagram after to rave about the performance, and to share an amazing shot of Lopez' mindblowing gown.
"I gotta say that #LinManuelMiranda's nerdom is the coolest thing to witness ever. It's so disarming even @JLo was in her most playful mood she's ever been in on our #FallonTonight set," Questlove captioned the pic. "We performed #LoveMakeTheWorldGoRound benefiting our brothers and sisters caught up in the Orlando tragedy. Great moment 2nite. Hope you guys watch it."
WATCH: Lin-Manuel Miranda Opens Up About Leaving 'Hamilton': 'This Is Not the End of Anything'
Proceeds from the sale of the song, which is available on iTunes, will go directly to the Somos Orlando Fund, which helps provide mental health care and services to the victims of the tragic shooting in Orlando last month.
Check out the video below for a look at Lopez and Miranda on the set of their "Love Make the World Go Round" music video.