A heckler and his friend were detained by authorities after shouting out "You are a rapist," in the middle of Cosby's set at Budweiser Gardens on Thursday in the Canadian city of London, Ontario.
"No one said anything," heckler Matt Smith told reporters outside the venue. "We were really surprised, because we're in Canada. We're a good country and we were expecting more from the people in there tonight. That's why we said something."
Dale Carruthers and Pat Maloney of The London Free Press were covering the event.
"I pulled out my phone and started filming them detain the two men," Dale said. "That's when arena officials told me to leave and security and police came over and escorted me out the front door."
This follows the backlash at Cosby's first Canada show on Wednesday, where protesters blocked the entrance to the Centre In The Square in Kitchener.
"Obviously any big change requires all kinds of voices, all kinds of action," protest organizer Melanie Baker said. "This is the one we chose."
During Thursday's performance in London, Ontario, several reports claim that the 77-year-old comedian made light of his recent sexual abuse allegations after he called out a woman who told him she was going to get a drink during his performance. He reportedly responded, saying, "You have to be careful about drinking around me."
Following Thursday's performance, Cosby released a statement to ET, saying, "Dear Fans: One outburst but over 2600 loyal, patient and courageous fans enjoyed the most wonderful medicine that exist for human-kind. Laughter. I thank you, the theatre staff (Budweiser Gardens), the event organizers and the London, ON community for your continued honor and support. I'm Far From Finished."