Madonna was the guest of honor on Thursday's The Tonight Show, and to kick things off, Fallon, the Material Girl and The Roots teamed up to perform a fun cover of her hit 1983 single "Holiday."
The whole group donned Hawaiian shirts and retro shades as they did their rendition of the classic tune on classroom instruments.
VIDEO: Madonna Gets Candid: Acting Her Age, Making Movies and Which Scorsese Film She Almost Starred In
Madonna really got into it, singing, whistling and wailing on a cowbell like the rock star fans know and love.
Performing covers of pop classics on classroom instruments has been a beloved recurring segment on The Tonight Show. Fallon and his crew have done this with Carly Rae Jepsen, Frozen's Idina Menzel, pop diva Mariah Carey, Robin Thicke and Meghan Trainor.
NEWS: Top 5 Favorite Jimmy Fallon Lip Sync Battles
Fallon isn't the first host to recently honor Madonna. Just this past March, Ellen DeGeneres celebrated Madonna Week, during which the singer and Justin Bieber played a racy game of Never Have I Ever. Check out the video to see their surprising responses.