She appeared in the first showcase of the night, rocking a silver, fringe-covered number with thigh-high lace-up boots, and shared a fun, beaming moment with fellow Victoria's Secret Angel Lily Aldridge.
During the show, some of the other models who walked during the event reflected on Ambrosio's legacy on the VSFS catwalk, and her impact on their own modeling careers.
"Alessandra Ambrosio is the ultimate Angel, and I think she will always be," Taylor Hill shared.
"She killed the runway for so many years," Nadine Leopold said. "She's a huge idol."
For her last true VSFS runway appearance, Ambrosio hit the catwalk during the evening's second performance by Miguel, rocking a bold, colorful, indigenous-inspired ensemble with giant, multi-colored feathers serving as her iconic Angel wings.
The cheers from the crowd grew louder as she walked to the front of the runway, where she silently mouthed "I love you forever" to the audience, while making a heart shape with her fingers, and waving to fans as she strutted off stage as the final model to walk during that portion of the show.