"When I was trying out to be centerfold, they actually give the girls five days of a test," McCarthy, 44, explained. "You shoot one position for five days."
"What was the position?" Andy Cohen interjected.
"I can imitate it," she said, striking a sexy pose while leaning against her chair.
McCarthy then elaborated on how Hefner criticized her sultry facial expressions.
"So eight hours a day for five days, and on day four, the producer said, 'Come meet with me, Hef is on the phone,'" she said. "And Hef said, 'You've got one more chance. Go home and practice, because your face looks like you just hit a deer with your car. You look so horrified.'"
The star broke down last month on her SiriusXM radio show, Dirty, Sexy, Funny With Jenny McCarthy, when addressing the publishing legend's death at 91 years old. McCarthy credited Hefner with kickstarting her career.
"It gave me on-camera experience, so by the time I auditioned for MTV, I was able to get that job," she recalled. "And through the numerous covers I did after that or the specials that I would host with Hef, I'll never forget."
McCarthy continued through a few sniffles and tears, "I just thought I wanted one more chance just to say thanks and thank you for sharing your dream with me, and for giving so many girls, and so many people, the opportunity of a lifetime."
For more on the star's reaction to Hefner's passing, watch the video below.