Madonna Spanks Amy Schumer at Concert While Jennifer Lopez Looks More '90s Than Ever!

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Amy Schumer may have opened for Madonna at Madison Square Garden on Wednesday night, but the real comedy came later on in the show when Madge brought the funny lady back to the stage during "Unapologetic Bitch."

For her encore, Amy got a little twerky for the GRAMMY winner, bending over in a short skirt while the Material Girl playfully spanked the comedian's backside.

WATCH: Amy Schumer Opening for Madonna

"Ma f**king Donna," Amy Instagrammed along with a picture of the epic moment.

Before the night was done, Madonna gifted Amy with a banana and posted an Instagram photo of the Trainwreck star accepting the present.

"Love you Amy!" Madonna wrote. "Bitch you ARE IN my gang! Thank You!! #rebelhearttour."

PICS: Madonna Is 56 and Not Showing It!

Amy will be opening for Madonna again at MSG tonight and at Brooklyn's Barclays Center on Saturday as a part of the New York leg of Madonna's Rebel Heart tour.

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The event brought out major star power as Jennifer Lopez and boyfriend Casper Smart were counted among the 20,000 fans at Wednesday's show, where J. Lo brought the '90s back in a flannel jacket and blue jeans. Andy Cohen and siblings Ariana and Frankie Grande were also in attendance.