Charlie Sheen telling his family may have been the hardest part.
After the 50-year-old actor revealed that he is HIV positive on Tuesday's Today, the former Two and a Half Men star revealed that he had broken the news to his oldest daughter, 30-year-old Cassandra Estevez, just days before.
"I told my oldest daughter Cassandra the other night, and I felt bad, it hit her hard," Sheen admitted. "But she recovered, and she’s tough like her dad."
WATCH: Charlie Sheen Reveals He's HIV Positive
"I said, 'Sweetie, I'm sorry I didn't tell you sooner, but it didn’t seem like you could do anything for me, and I didn’t want to burden you with the stress," he continued. "But she was a rock star about it."
Sheen has one granddaughter, Jade, through his oldest daughter, who was born in 1984 to the actor's high school girlfriend, Paula Profit.
The actor also revealed that his ex-wives, Denise Richards and Brooke Mueller, were previously made aware of his diagnosis. Mueller was actually still married to Sheen in 2011, when he found out about his diagnosis. Sheen said that he "absolutely" told Mueller right away so that she could get checked.
WATCH: Source: Denise Richards and Her Kids Not HIV Positive
Following the actor's interview, Mueller's rep, Steve Honig, issued a statement to ET.
"Brooke has been inundated with calls from friends and family," he said. "To put their minds at ease, Brooke can confirm that she and the boys are not HIV positive."
Meanwhile, a source told ET on Monday that Richards and her two daughters with Sheen, 11-year-old Sam and 10-year-old Lola, are also HIV negative, adding that Sheen got infected after he and Richards split in 2006. Two weeks ago, Richards appeared on the Allegedly podcast with Theo Von, where she said, "Charlie, when I married him, he wasn’t a bad boy. He was sober for almost four years. He was a very different person. His lifestyle was very, very different. He was a former bad boy, I guess."
WATCH: Bree Olson Claims She Learned of Charlie Sheen HIV Diagnosis Via Media
Sheen dated former adult film actress, Bree Olson, in 2011, who said on Howard Stern's Sirius XM radio show after his interview, that Sheen never told her about his diagnosis.
"He never said anything to me," Olson told Stern. "We had sex almost every day for a year."
The 29-year-old lived with Sheen in 2011, and reportedly left him in April of that year. Sheen said in his interview that it was "impossible" he had "knowingly or even perhaps unknowingly transmitted the HIV virus to someone else." Sheen said he’s had unprotected sex with two people since he was diagnosed, and both knew of his condition and were under treatment of his doctor.
WATCH: Brooke Mueller Confirms She and Kids Are HIV Negative After Charlie Sheen Interview
Despite his diagnosis, Sheen's doctor said in a statement to ET that the actor actually has "undetectable" levels of the virus in his blood.
"Four years ago, Charlie Sheen came to see me with an upper viral infection followed by joint aches, swallowing complaints and then progressive severe headaches," Robert Huizenga said. "During this infection, his HIV tests 'seroconverted' (acutely changed) from negative to positive. He was immediately placed on four antiretroviral drugs and his HIV viral blood levels thankfully dropped to undetectable. He has tolerated his antiretroviral cocktail and by faithfully complying with the daily pills, his viral HIV load has remained consistently undetectable."
"With Charlie remaining dedicated to his treatment regimen, I expect the HIV will only minimally -- if at all -- affect his predicted life expectancy," he added.
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On the Today show, Huizenga also stressed that Sheen does not have AIDS.
"AIDS is a condition when the HIV virus markedly suppresses the immune system and you are susceptible to rare difficult cancers and infections. Charlie has none of those," he explained. "He is healthy. He does not have AIDS."
MORE: Charlie Sheen Pens Open Letter About Being HIV Positive: 'My Partying Days Are Behind Me'
"I anticipate Charlie can save many more lives coming forward with his revelation than I could ever have aspired to as a doctor," he said in his statement.
For his part, Sheen revealed in an open letter obtained by ET that his "partying days are behind" him.
Watch more of the actor's HIV admission below.