Other celeb cameos in the performance ultimately also included the likes of supermodel and "Toothbrush" video co-star Ashley Graham, New York Giants wide receiver Odell Beckham Jr., rappers A$AP Ferg and Fabolous and Instagram stars Joanne the Scammer and The Fat Jewish as well as the single's featured artist, Ty Dolla $ign.
Ahead of the awards show, Nick teased to ET on the white carpet that there would be "some special brother bonding, in a sense."
It was a special night for the brothers. In addition to Nick's star-studded performance, Joe's band DNCE was nominated -- and won -- the Best New Artist category.
"It's the best feeling in the world," the 23year-old singer said of sharing the night with Joe. "It's really unique to be in a spot where we're both here separately but also together, in a sense. I'm proud of [DNCE]. This year they've had has been unbelievable."
And will we see more collaboration between the two younger Jonases in the future?
"Never count anything out," Nick said. "We obviously like each other quite a bit. We're roommates. We just got matching tattoos."
Yep, the JoBros got inked together! Nick posted a pic of the new body art to Snapchat, which showed two arrows on the back of their arms that point towards each other.