Before accepting the most prestigious award of the night -- the Michael Jackson Video Vanguard Award -- the 49-year-old singer took the stage at the MTV Video Music Awards to perform a medley of hits spanning her impressive 20 years in the spotlight.
Lopez, clad in a flowy dress, kicked off her performance at New York City's Radio City Music Hall with her 1999 hit, "Waiting for Tonight," briefly suspended in the air and backed by a group of sexy (and shirtless!) male dancers.
She then went straight into "On the Floor," later taking off her dress to reveal a stunning, gold bodysuit as she danced to "Dance Again."
Her female dancers hit the stage for an epic dance break to "Ain't Your Mama" and "Booty," followed up by "My Love Don't Cost a Thing," which featured some major booty shaking.
But that's not all! She continued to bring the fire with a medley of a few of her most classic hits, "I'm Glad" and "Get Right."
And paying homage to the iconic music video, J. Lo stepped into a white fur coat to sing the chorus of "All I Have" as the cameras panned to her adorable 10-year-old daughter, Emme.
The best part of Lopez's performance, however, really began when DJ Khaled came out to join her onstage. Singing and dancing in front of a New York City-themed graffiti background that read "Jenny From the Block," the Bronx native belted out her 2002 hit after a quick change into a glittery gold jumpsuit, complete with her signature New York Yankees hat.
Her frequent collaborator, Ja Rule, then hit the stage to sing their remixed hits, "I'm Real" and "Ain't It Funny," before Lopez finished the performance with "Dinero."
Interestingly enough, this is the first time Lopez has performed at the VMAs since 2001, when she brought Ja Rule onstage to sing "I'm Real":
While accepting her Video Vanguard Award, Lopez thanked her family, friends and boyfriend, former MLB star Alex Rodriguez.
"It has been a crazy journey, dreaming my dreams and watching them come true," she said. "This career has always been kind of an obsession for me."
In honor of Lopez's big night at the VMAs, ET recently took a trip down memory lane. Take a look back at her best music videos of all time here and in the video below: