Perry, who was best known as Dylan McKay on Beverly Hills, 90210, was featured in one of the night's Best Picture nominees. Once Upon a Time in Hollywood was his final onscreen appearance before his shocking death last March following a massive stroke. He was 52.
Among those honored in the heartfelt segment were NBA legend and Los Angeles legend Kobe Bryant, who died in a tragic helicopter crash in Calabasas, California -- along with his 13-year-old daughter, Gianna, and seven others, on Jan. 26. He was 41.
On top of Bryant's five NBA championship rings, the celebrated star also took home an Oscar for Best Animated Short Film for Dear Basketball, which Bryant wrote and narrated. The win made him the first African American to win an Oscar in that category, and the first professional athlete ever to be nominated and win an Oscar in any category at all.
The emotional tribute also remembered a number of other past Oscar nominees, including the legendary producer of Chinatown and The Godfather, Robert Evans, screenwriter Buck Henry -- best known for penning The Graduate and Catch-22 -- Cross Creek and Men in Black star Rip Torn, Blade Runner star Rutger Hauer, French documentarian and Faces Places director Agnes Varda, as well as Pillow Talk actress and singer Doris Day.
Celebrated filmmaker John Singleton was also honored in the emotional video package. Singleton -- who died on April 28 at the age of 51 after suffering a stroke -- made Oscars history in 1992 when he became both the first African American filmmaker and the youngest person ever to be nominated for Best Director at the Oscars for his film, Boyz n the Hood. His work in the groundbreaking drama also earned him a nomination for Best Original Screenplay.
Among the many other actors whose lives and legacies were fondly remembered were Luke Perry, Robert Forster, comedy icon Tim Conway, Monty Python's Terry Jones, Danny Aiello, Diahann Carroll, Peggy Lipton and Peter Mayhew, among many others.
For more on this year's emotional, surprising and exciting Oscars ceremony, check out the video below.