Casually dressed in black pants and white tee, Payne also sported a necklace with a bear charm, which was given to him on Father's Day by his girlfriend, Cheryl Cole, as a way to always keep his newborn son, Bear, close to him.
During his performance, the singer got down and showed off his moves alongside a group of backup dancers.
Payne also played "Secret Ingredient" with Fallon, Ashton Kutcher and Vanessa Hudgens. The stars had to eat a special dish and guess the unusual secret ingredient hidden in the meal.
Earlier this week, the former One Directoner was also part of the charity fundraising single, "Bridge Over Troubled Water, a cover of Simon & Garfunkel's hit, that was released to benefit the victims of the fire at London's Grenfell Tower.