The actor took home the award for Hero of the Year for his performance as Sam Wilson in The Falcon and the Winter Soldier, and he got a bit emotional while sharing his appreciation on stage at the Palladium in Los Angeles.
"Everything that happened in the past year and a half has been literally a... a very hard experience," Mackie said, choosing his words delicately, and with a sadness in his voice. "But we've gotten through and we are stronger for it."
"Thank you, MTV, for this. It means a lot," he continued. "You guys will never know the amount of blood, sweat, and tears that went into this show. So, thank you."
Later in the evening, Mackie took to the stage once again when he and his friend and co-star Sebastian Stan took home the Best Duo award for their roles in the hit Marvel series.
Mackie took to the stage solo and explained that Stan was unable to attend because he's busy filming his Tommy Lee biopic.
"Sebastian couldn't be here because he's off being a rock star," Mackie said, smiling. "It's really amazing because he's one of my dear friends, and the two of us really enjoyed doing this show."
"We had a great time, we jogged together, we ate a lot of protein together. We laughed at each other," he recalled. "He locked himself in his room and I banged on the door and finally stole all of his luggage so he had to come out of his room to get clean clothing. But it was a great time and I'm so happy and I appreciate so much the fact that you guys loved the show so much. There's more to come."
Before the show, ET's Katie Krause caught up with Mackie on the red carpet and the actor expressed his excitement for the return of in-person awards shows and the very presence of red-carpet interviews.
"We're still in masks. But it's great," he shared. "The whole idea of just being back into some semblance of reality is really great."
"Yo, I'm not trying to brag, but my boy looks good! Like, when I heard he was playing Tommy Lee, I was like, 'Eh, I don't know what to think about this, Sebastian. You bit off more than you can chew.' But I saw the pictures, and my boy killed it," Mackie marveled. "Tommy Lee should be proud... He looks amazing! Put in the work, he did the time, he looks phenomenal. So I'm excited to see it."