"I want to say hi to my folks because, hey," he joked with his arms out wide, as the audience laughed. "They're back in the Ozarks. I wanted to bring my mom, but everyone I sit next to they say I'm dating."
Lastly, Pitt concluded with a sweet message.
"If you have the chance to be kind tomorrow, take it," he said. "I think we need it."
Pitt is a nine-time Golden Globes nominee, and previously won the award for Best Supporting Actor in 1996, for his role in 12 Monkeys. He also took home a Globe as a producer in 2014 for 12 Years a Slave, which won Best Motion Picture - Drama.
His acclaimed role in Once Upon a Time came opposite another A-lister, Leonardo DiCaprio, who played Rick Dalton, a veteran actor facing down the end of his career, who confides in Booth, his longtime friend, assistant and stuntman.
“I think we're all aware there's a shelf life on this,” Pitt said of his own career during a Today interview with NBC’s Harry Smith in July. “But it's really important to me the people I spend my time with and the artists that I respect.”
The win is the first big step toward the Best Supporting Actor Oscar at this year's Academy Awards on Feb. 9. Check out all of ET's awards season coverage and see more on the 2020 Golden Globes in the video below.